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- W2381398423 abstract "Objective:To screen and identify the gene clones from the cDNA library of the adult Paragonimus westermani (PwA) for immunodiagnosis and immunoprophylaxis. Methods:PwA cDNA library was screened with the PwA antigen immunized rabbits′ sera(IRS), which was pre absorbed by the extract E. coli XL1 Blue. Positive clones were amplified with PCR, the PCR products were identified by electrophoresis, and automatically excised in the help phages. The positive colony, which had recombinant plasmids, was screened on antibiotic containing plate. Two obtained positive clones named Pw 2 and Pw 4 were sequenced and the homology of their DNA sequence was analysed with BLAST software. Results:Two gene clones Pw 2 and Pw 4 were obtained, the length of their insert fragments were both 801bp and 1053bp respectively. Pw 2 and Pw 4 were homologous with Pw pre procathepsin L gene and Pw cysteinase gene respectively. The homology of them was 99% and 86%, seperatively. Conclusion:To screen PwA cDNA library with IRS firstly reported. Pw 2 and Pw 4 may apply to immunodiagnosis and immunoprophylaxis of paragonimiasis." @default.
- W2381398423 created "2016-06-24" @default.
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- W2381398423 date "2001-01-01" @default.
- W2381398423 modified "2023-09-25" @default.
- W2381398423 title "Immunoscreening of Paragonimus westermani Adult cDNA Library Using Polyclonal Antibody" @default.
- W2381398423 hasPublicationYear "2001" @default.
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