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- W2381400198 abstract "A pare peptide from dog brain is obtained. The whole dog brain was homogenized and extracted with 0.1M acetic acid. The extract was centrif aged at 15000g for 30 min. The supernatant was lyophilized and the residue was dissolyed in 0.1M acetic acid and was allowed to go through Sephadex G-50 column (twice), CM22 cellulose and Sp-Sephadex C-25 ion exchange column. The sample obtained was applied to a HPLC (0.5×30cm) with cross linking (3-10μ). The sample was called 74a peptide, and shows only one band on SDS-polyacrymid gel electrophoresis or isoelectric focusing. Its isoelectric point was around pH 7.5, and M. W. was about 10000 dalton. The sequince of the N-terminal 35 amino acid residue has determined. The 74a peptide show of a sinificant anti-morphine activity,This is a new peptide." @default.
- W2381400198 created "2016-06-24" @default.
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- W2381400198 date "1990-01-01" @default.
- W2381400198 modified "2023-09-25" @default.
- W2381400198 title "Method of the Isolation, Purification of an Anti-morphine Peptide (74a) from Dog Brain and Its Identification" @default.
- W2381400198 hasPublicationYear "1990" @default.
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