Matches in SemOpenAlex for { <https://semopenalex.org/work/W2381427931> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2381427931 abstract "Objective To observe the changes in Bax and Bcl-2 expressions and to investigate the biology role of estrogen receptor(ER)β1 in MDA-MB-231 breast cancer cells by using RNA interference to interrupt ERβ1 expression.Methods pSilencer-ERβ1 was transfected into MDA-MB-231 breast cancer cells by using cationic liposome Lipofectamine 2000TM as tranfecting agent.After transfection,the expression levels of mRNA and protein of ERβ1,Bcl-2 and Bax were evaluated by real-time polymerase chain reaction(RT-PCR)and Western blot,respectively.Cell apoptosis was detected using flow cytometry.Cell growth curve was performed to show the changes in proliferation ability.Comparison between quantitative data was performed sing t test and one factor analysis of variance.Results After ERβ1 siliencing,both mRNA and protein level of ERβ1 and Bax gene decreased significantly compared with the control groups(P0.05),and cell growth curve showed a marked enhancement in cell proliferation(P0.05).In the pSilencer-ERβ1 transfection group,there was an obvious decrease in mRNA and protein level of Bax gene(P0.01);no significant change in the expression of Bcl-2;and the ratio of Bcl-2/Bax increased.The results from flow cytometry showed that cell apoptosis decreased markedly in the pSilencer-ERβ1 transfection group compared with the controls(t=6.22,P=0.00).Conclusion ERβ1 could induce apoptosis of MDA-MB-231 breast cancer cells by regulating Bax gene expression,which may play an important role in ERβ1 inhibiting the growth of MDA-MB-231 breast cancer cells." @default.
- W2381427931 created "2016-06-24" @default.
- W2381427931 creator A5054299548 @default.
- W2381427931 date "2011-01-01" @default.
- W2381427931 modified "2023-09-23" @default.
- W2381427931 title "Estrogen receptor β1 induces apoptosis of MDA-MB-231 breast cancer cells by regulating the expression of Bax gene" @default.
- W2381427931 hasPublicationYear "2011" @default.
- W2381427931 type Work @default.
- W2381427931 sameAs 2381427931 @default.
- W2381427931 citedByCount "0" @default.
- W2381427931 crossrefType "journal-article" @default.
- W2381427931 hasAuthorship W2381427931A5054299548 @default.
- W2381427931 hasConcept C104317684 @default.
- W2381427931 hasConcept C121608353 @default.
- W2381427931 hasConcept C153911025 @default.
- W2381427931 hasConcept C185592680 @default.
- W2381427931 hasConcept C190283241 @default.
- W2381427931 hasConcept C40767141 @default.
- W2381427931 hasConcept C49923114 @default.
- W2381427931 hasConcept C502942594 @default.
- W2381427931 hasConcept C530470458 @default.
- W2381427931 hasConcept C54009773 @default.
- W2381427931 hasConcept C54355233 @default.
- W2381427931 hasConcept C553184892 @default.
- W2381427931 hasConcept C55493867 @default.
- W2381427931 hasConcept C62112901 @default.
- W2381427931 hasConcept C81885089 @default.
- W2381427931 hasConcept C84606932 @default.
- W2381427931 hasConcept C86803240 @default.
- W2381427931 hasConcept C92087593 @default.
- W2381427931 hasConceptScore W2381427931C104317684 @default.
- W2381427931 hasConceptScore W2381427931C121608353 @default.
- W2381427931 hasConceptScore W2381427931C153911025 @default.
- W2381427931 hasConceptScore W2381427931C185592680 @default.
- W2381427931 hasConceptScore W2381427931C190283241 @default.
- W2381427931 hasConceptScore W2381427931C40767141 @default.
- W2381427931 hasConceptScore W2381427931C49923114 @default.
- W2381427931 hasConceptScore W2381427931C502942594 @default.
- W2381427931 hasConceptScore W2381427931C530470458 @default.
- W2381427931 hasConceptScore W2381427931C54009773 @default.
- W2381427931 hasConceptScore W2381427931C54355233 @default.
- W2381427931 hasConceptScore W2381427931C553184892 @default.
- W2381427931 hasConceptScore W2381427931C55493867 @default.
- W2381427931 hasConceptScore W2381427931C62112901 @default.
- W2381427931 hasConceptScore W2381427931C81885089 @default.
- W2381427931 hasConceptScore W2381427931C84606932 @default.
- W2381427931 hasConceptScore W2381427931C86803240 @default.
- W2381427931 hasConceptScore W2381427931C92087593 @default.
- W2381427931 hasLocation W23814279311 @default.
- W2381427931 hasOpenAccess W2381427931 @default.
- W2381427931 hasPrimaryLocation W23814279311 @default.
- W2381427931 hasRelatedWork W1487834820 @default.
- W2381427931 hasRelatedWork W2066878200 @default.
- W2381427931 hasRelatedWork W2162820194 @default.
- W2381427931 hasRelatedWork W2248992977 @default.
- W2381427931 hasRelatedWork W2348193306 @default.
- W2381427931 hasRelatedWork W2363067705 @default.
- W2381427931 hasRelatedWork W2364309381 @default.
- W2381427931 hasRelatedWork W2367169678 @default.
- W2381427931 hasRelatedWork W2367404456 @default.
- W2381427931 hasRelatedWork W2372715335 @default.
- W2381427931 hasRelatedWork W2390858379 @default.
- W2381427931 hasRelatedWork W2392572192 @default.
- W2381427931 hasRelatedWork W2393301686 @default.
- W2381427931 hasRelatedWork W2393375070 @default.
- W2381427931 hasRelatedWork W3032631381 @default.
- W2381427931 hasRelatedWork W3113910931 @default.
- W2381427931 hasRelatedWork W3148256673 @default.
- W2381427931 hasRelatedWork W2414797142 @default.
- W2381427931 hasRelatedWork W2570281235 @default.
- W2381427931 hasRelatedWork W2743629035 @default.
- W2381427931 isParatext "false" @default.
- W2381427931 isRetracted "false" @default.
- W2381427931 magId "2381427931" @default.
- W2381427931 workType "article" @default.