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- W2381429603 abstract "According to the sequence of the genome of the Beaudette strain of IBV, and with a gene analysis program, three primers (you1+、you2- and youM+) were designed and synthesized. The primers you1+ and you2-were located respectively in the 5'most and the 3'most end of the S1 gene and approximately cover 1 611 bp and the primer youM+ lying in the middle of the S1 gene. RTPCR was used to amplify the S1 gene fragment of the five Chinese IBV isolates(HN2,HN4,JX1,SC2 and SC4) using the primers you1+ and you2-. The result indicated that the length of the RTPCR products was the same as expected. A sequence of 535 bp was amplified by PCR using the primers you2- and youM+ when every RTPCR product of the five IBV isolates was used for template. The RTPCR products were digested with the restriction enzyme PstⅠ and two fragments (560 bp and 1 050 bp) were seen, which were the same for the 11 published S1 sequences of IBV strains in Genebank. Therefore , all these results demonstrate that the S1 gene fragments of the five IBV isolates were isolated successfully." @default.
- W2381429603 created "2016-06-24" @default.
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- W2381429603 date "2001-01-01" @default.
- W2381429603 modified "2023-09-25" @default.
- W2381429603 title "The Amplification of the S1 Gene of Five Chinese Avian Infectious Bronchitis Virus Isolates and Identification" @default.
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