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- W2381439293 abstract "Objective To approach a series of parameters of denitrification engineering bacteria PNS in wastewater treatment.Methods The recombination engineering bacteria pQE30-nirS-SG13009(PNS) was constructed with the technology of gene engineering which forced cloning nirS gene into expression vector pQE-30.Then the optimal induction concentration and time of PNS were analyzed by SDS-PAGE.Following,the PNS which was induced in anaerobic condition and at 20 ℃ was broken into pieces and centrifuged in order to get its sediment and supernatant,and then the expression form of cd1-nitrite reductase was analyzed by SDS-PAGE.Results The optimal induction concentration of IPTG was 0.05 mmol/L,the optimal induction time of IPTG was 2 hours,the PNS could express dissoluble cd1-nitrite reductase in anaerobic condition and at 20 ℃.Conclusion The denitrification engineering bacteria PNS can express cd1-nitrite reductase in wastewater treatment which possesses biologic activity." @default.
- W2381439293 created "2016-06-24" @default.
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- W2381439293 date "2008-01-01" @default.
- W2381439293 modified "2023-09-23" @default.
- W2381439293 title "Preliminary study on denitrification engineering bacteria PNS in wastewater treatment" @default.
- W2381439293 hasPublicationYear "2008" @default.
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