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- W2381448129 abstract "Objective To construct IL-18 gene in human umbilical cord mesenchymal stem cells for exploring a means of tumor targeted gene therapy. Methods hUMSCs were isolated and cultured in vitro.The immunophenotype of hUMSCs was detected by flow eytometry.The lentivirus vector containing human IL-18 gene was constructed and transfected into hUMSCs.The IL-18 mRNA and protein expression level was detected by semi-quantitative RT-PCR and Western blot. Results hUMSCs were isolated and cultured successfully.The FACS indicated that CD29,CD44 and CD105were positive,and the CD34 and CD45were negative,fitting the phenotype of hUMSCs.IL-18-hUMSCs was created successfully.RT-PCR and Western blot showed that IL-18 gene was transfected into hUMSCs and stably expressed. Conclusion hUMSCs that carries and stably expresses gene IL-18 has been created,which provides a new method for experimental research of targeted tumor gene therapy." @default.
- W2381448129 created "2016-06-24" @default.
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- W2381448129 date "2014-01-01" @default.
- W2381448129 modified "2023-09-24" @default.
- W2381448129 title "CONSTRUCTION OF IL-18 GENE IN HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS" @default.
- W2381448129 hasPublicationYear "2014" @default.
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