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- W2381461172 abstract "Objective To investigate the effect of Golgi body vesicular transporter P115 gene silencing on apoptosis of gastric cancer BGC-823 cells as well as the relevant mechanism.Methods BGC-823 cells were transfected with recombinant plasmid p115 shRNA-1318 containing p115 gene,setting negative control(shNC) and blank control groups at the same time,and determined for transfection efficacy by IFA,for transcriptions of p115 and macrophage migration inhibitory factor(MIF) mRNAs by RT-PCR,for expression levels of P115,MIF and intranuclear tumor-inhibiting protein P53 by Western blot,for proliferation activity by MTT method,and for apoptosis and distribution of cell cycles by flow cytometry.Results The expression of green fluorescent protein(GFP) was observed in BGC-823 cells 48 h after transfection,indicating a successful transfection.Compared with those in control groups,both the mRNA transcription levels and protein expression levels of p115 and MIF genes in the cells transfected with recombinant plasmid p115 shRNA-1318 decreased significantly(P 0.05),while the expression level of intranuclear P53 protein increased significantly(P 0.05);the proliferation activity of BGC-823 cells transfected with p115 shRNA-1318 decreased significantly(P 0.05),while the apoptosis level increased significantly(P 0.05);however,the number of cells transfected with p115 shRNA-1318 at G1 / G2 phases increased significantly,while that at S phage decreased significantly(P 0.05).Conclusion The p115 gene silencing promoted the apoptosis of gastric cancer cells by a potential mechanism of regulating MIF." @default.
- W2381461172 created "2016-06-24" @default.
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- W2381461172 date "2012-01-01" @default.
- W2381461172 modified "2023-09-23" @default.
- W2381461172 title "Effect of Golgi body vesicular transporter P115 gene silencing on apoptosis of gastric cancer cells and relevant mechanism" @default.
- W2381461172 hasPublicationYear "2012" @default.
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