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- W2381522556 abstract "In order to isolate expeditiously the HIV1U26942 DNA fragments for preparation of DNA microarrays, the multiple gene fragments with sizes suitable for DNA microarrays, produced by digesting the dissociated HIV gene with Sau 3AⅠ, were ligated with universal adapters. PCR primers were designed to match the universal adapters (including the restriction site sequence) but with one nesting base overhanging at the 3' end. The PCR reactions that were performed with various single primers or primer combinations were divided into ten subgroups. PCR products were purified and then cloned into the T vectors. The positive clones were propagated and the plasmids were extracted. The target HIV gene fragments were isolated and sequenced, which were correlated precisely with the prediction of restriction analysis. Eighteen gene fragments ranging from 0.1kb to 1kb were prepared for DNA microarray. Restriction display is an effective and rapid method for the isolation of gene fragments" @default.
- W2381522556 created "2016-06-24" @default.
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- W2381522556 date "2002-01-01" @default.
- W2381522556 modified "2023-09-25" @default.
- W2381522556 title "Preparation of Gene Fragments from HIV-1 U26942 by Restriction Display" @default.
- W2381522556 hasPublicationYear "2002" @default.
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