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- W2381529391 abstract "Considering that ramie is rich in secondary metabolites such polyphenols,sticky substances and flavone,the optimized CTAB protocol was screened for extracting the total DNA of ramie.The addition of polyvinylpyrrolidone(PVP) during the grinding and the additions of Polyvinylpyrrolidone at the final concentration of 6% and β-mercaptoethanol at the final concentrations of 4% to the extraction buffer were capable of preventing browning from taking place in the extraction;while chloroform/ isoamyl alcohol were used to conduct the second extraction,10% CTAB and 4% NaCl were added to remove polysaccharides.The total DNAs extracted by the several modified CTAB protocols were amplified by ISSR-PCR and the amplification proved that CTAB-4 in which both the antioxidant and the salt at high concentration were added performed best." @default.
- W2381529391 created "2016-06-24" @default.
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- W2381529391 date "2005-01-01" @default.
- W2381529391 modified "2023-09-26" @default.
- W2381529391 title "Optimized CTAB Protocol for Extracting the Total DNA of Ramie" @default.
- W2381529391 hasPublicationYear "2005" @default.
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