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- W2381567680 abstract "Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll density gradient centrifugation of heparinized blood,and PBMC were plated into 6 well plates for 3 hour incubation The non adherent cells were removed and the adherent cells (monocytes) were cultured with GM CSF (100 ng/ml) and IL 4 (50 ng/ml) for 5 days Then TNF α (10 ng/ml) or recombinant human soluble CD40 ligand (rhsCD40L) (2 μg/ml) was respectively added to the cultures for another 4 days' induction Cell morphology was monitored by light microscopy and cell surface molecules including CD1α,CD80,CD83,and HLA DR by flow cytometry The induced DC functions were estimated by detecting the capacities of DC up take antigens with FITC Dextran capturing assay,of stimulating autogenetic lymphocyte in vitro proliferation with 3 H TdR insertion method,of chemotaxying T lymphocyte with Trans well and of secreting IL 12 with ELISA It was found that rhsCD40L (2 μg/ml) successfully developed peripheral blood monocytes into mature DC combined with GM CSF and IL 4,which with the typical morphology,special surface markers like CD1α,CD80,CD83,and HLA DR,with the full capacities of uptaking FITC Dextran particle,stimulating autogenic lymphocytes to proliferation,driving T lymphocytes to migration and IL 12 secretion Moreover,our results demonstrated that the expression levels of some immune co stimulators on DC including CD80 and HLA DR were higher than those DC induced with GM CSF+IL 4+TNF α Also the rhsCD40L derived mature DC expressed higher levels of CXCR4 and attracted more number of T lymphocytes than DC induced with TNF α or FL These results suggest that rhsCD40L has the potential role in stimulating the differentiation and maturation of monocyte derived DC and may be a more efficient stimulator for T lymphocytes" @default.
- W2381567680 created "2016-06-24" @default.
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- W2381567680 date "2002-01-01" @default.
- W2381567680 modified "2023-09-25" @default.
- W2381567680 title "Recombinant Human Soluble CD40 Ligand Exerts Powerful Lmpacts on the Development and Functions of Monocyte-derived DC in Vitro" @default.
- W2381567680 hasPublicationYear "2002" @default.
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