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- W2381604350 abstract "This article reports the cell survival and growth of Porphyra haitanensis after different cold storage time using different cryoprotectants,and discusses some correlative factors which may affect storage effect.Results are as follows:(1)In normal temperature,there was lower cell survival of P.haitanensis at different concentrations of Glycerin and DMSO than that of Glucose and Sucrose.P.haitanensis were cultured in 20% DMSO and 35% Glycerin,and the survival rates were 15% and 0%,respectively.(2)Refrigerated in Glycerin,it had higher survival rate than at the same concentration of DMSO.Refrigerated in Glycerin for 30 d,the survival rate was 100%;refrigerated in Glycerin for 90 d,the survival rate was 25%35%.Refrigerated in Glucose and Sucrose for 15 d,the survival rate was 0%.(3)Refrigerated in combination of DMSO and Glycerin for 30 d,the survival rate was 85%.(4)Different combination of DMSO,Glycerin and Glucose showed relatively good refrigeration effect.After 180 d refrigeration,the cell survival rate got to 95%.(5)Salinity and thickness of cell wall had some bearing on refrigeration effect.When salinity was 25,the cell viability was comparably higher than that at other salinity.The thickness of cell wall had some impact on the storage effect.In the same strain,the cells with thick cell wall possessed stronger low-temperature resistance and higher survival rate than those with thin cell wall refrigerated for 30 d.(6)In the different part of the same plant,some difference of survival rate also existed after refrigeration for 30 d." @default.
- W2381604350 created "2016-06-24" @default.
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- W2381604350 date "2007-01-01" @default.
- W2381604350 modified "2023-09-23" @default.
- W2381604350 title "Effects of cryoprotective agent on cold storage of Porphyra haitanensis larva" @default.
- W2381604350 hasPublicationYear "2007" @default.
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