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- W2381610303 abstract "Aim: To observe the biological changes of A549 cells after esp1 gene transfection.Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated.Apoptosis was analyzed by Annexin V-FITC method, and proliferation was detected. Cell colony formation in soft agar was observed as well. Cells transfected IRE-EGFP (neo-A549) and without transfection (A549) were the control. Results: Compared with neo-A549 and A549, DI,SPF, and proliferation of esp1-A549 cells significantly decreased (P0.05), while apoptosis increased(P0.05).The ability of colony formation in soft agar of esp1-A549 cells was suppressed compared with A549( P0.05).Conclusion: Up-regulation of esp1 can inhibit the malignant biological behavior of A549 cells." @default.
- W2381610303 created "2016-06-24" @default.
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- W2381610303 date "2006-01-01" @default.
- W2381610303 modified "2023-09-26" @default.
- W2381610303 title "Biological behavior of A549 cells after esp1 gene transfection" @default.
- W2381610303 hasPublicationYear "2006" @default.
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