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- W2381652016 abstract "[Objectives] To further understand the molecular mechanisms underlying the overexpression of the Ras1 CAoncogene in the posterior silk gland(PSG) of Bombyx mori and how this improves silk yield at the transcriptional level. Transcriptomic data from differentially expressed genes(DEGs) in the pathway of Ras1CA-overexpressed and wild type PSG were analyzed and verified by quantitative real-time PCR(q RT-PCR). [Methods] Deep RNA sequencing of silkworm PSG was carried out and KEGG pathway enrichment analysis was conducted on the DEGs. The DEGs in the pathway was selected to be verified by q RT-PCR. [Results] RNA-Seq revealed 2 636 DEGs in Ras1CA-overexpressed PSG compared to wild type PSG. There were 42 DEGs distributed in the cell cycle pathway, including cyclin dependent kinases(CDKs), cyclins and transcription factors. Consistent with the transcriptomic data, q RT-PCR verification of the selected genes; cdk1, cyclin D1, cyclin D2, cdc7, cdh1, dp-1,2, indicated that all of these were significantly upregulated by Ras1 CA. [Conclusion] Transcriptomic analysis revealed that there are a number of DEGs in Ras1CA-overexpressed and wild type PSG. Ras1CA-overexpression upregulates some DEGs in the pathway that benefit silk gland growth and fibroin synthesis. These results advance our knowledge of the molecular mechanism underlying how Ras1CA-overexpression in the PSG improves fibroin production and silk production." @default.
- W2381652016 created "2016-06-24" @default.
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- W2381652016 date "2015-01-01" @default.
- W2381652016 modified "2023-09-26" @default.
- W2381652016 title "RNA-Seq technology based transcriptomic analysis of differentially expressed genes in the cell cycle pathway of Ras1~(CA)-overexpressed and wild type posterior silk glands of Bombyx mori" @default.
- W2381652016 hasPublicationYear "2015" @default.
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