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- W2381657315 abstract "Objective To construct humanize phage antibody library against Ascaris lumbricoides and to screen specificity scFv to Ascaris lumbricoides.Methods Total RNA was abstracted from peripheral blood lymphocytes of 20 persons,and was used to amplify VH and VL gene by RT-PCR.The amplified VH and VL genes were spliced to form scFv gene which was cloned into pCANTAB-5E phagemid,and the constructed recombinant phagemid was transformed to E.coli TG1 to construct human natural single-chain antibody library from which positive clones were screened.Results A primary library of 1.5×106 and a second library of 1.2×106 were constructed.Conclusion This study was to provide us the basis for radionuclide imaging and therapy for ascariasis." @default.
- W2381657315 created "2016-06-24" @default.
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- W2381657315 date "2011-01-01" @default.
- W2381657315 modified "2023-09-23" @default.
- W2381657315 title "Preparation and Identification of a Human Single Chain Fv Antibody Against Ascaris lumbricoides" @default.
- W2381657315 hasPublicationYear "2011" @default.
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