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- W2381665123 abstract "The etiolated seedlings of Leymus multicaulis were homogenized by grinding in liquid nitrogen.The nuclei were isolated by suspending the powder in ice-cold nuclei isolation buffer,filtering by a series of steeliness cell griddle on ice and centrifuging before photographed under phase-contrast microscope and embedded in low melting point(LMP) agarose.After digestion with proteinase K in situ and isolation by pulsed-field gel electrophoresis,pure HMW nuclear DNA over 2 Mb was isolated.Southern hybridization indicated there was no protoplast and mitochondria DNA contamination.The HMW nuclear DNA prepared using this method was suitable for transformation artificial chromosome(TAC) library and genome analysis of L.multicaulis" @default.
- W2381665123 created "2016-06-24" @default.
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- W2381665123 date "2007-01-01" @default.
- W2381665123 modified "2023-09-28" @default.
- W2381665123 title "An efficient protocol for extraction of high molecular weight nuclear DNA from Leymus multicaulis" @default.
- W2381665123 hasPublicationYear "2007" @default.
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