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- W2381690723 abstract "TasA is a protein produced during sporulation of Bacillus subtilis.It has strong inhibitory effect to many animal and plant pathogens.The full-length sequence of TasA gene was cloned from the genomic DNA of mulberry endophytic bacterium Bacillus subtilis strain ME0717(GenBank accession number FJ713582).The full length DNA sequence includes a complete ORF of 786 base pairs.Compared with other TasA sequences previously reported,the gene had a high sequence similarity with those of B.subtilis,which was 99.0% with Z99116 and 98.1% with AJ871386,while the similarity with the sporulation-associated gene YqhF(GenBank accession number BACJH642)reached 99.0% as well.Sequence similarity with TasA gene of B.licheniformis(GenBank accession number AE017333)was only 46.0%.The gene also has a high level similarity with the genes of cell envelope-bound metalloprotease and spore coat-associated protein from Bacillus.The prokaryotic expression vector carrying TasA gene was constructed and expressed in Escherichia coli BL21.The expressed protein showed certain inhibitory activity on growth of bacterial cells of Pseudomonas syringae pv.mori and of mycelia of Colletotrichum morifolium and Myrothecium roridum." @default.
- W2381690723 created "2016-06-24" @default.
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- W2381690723 date "2010-01-01" @default.
- W2381690723 modified "2023-09-25" @default.
- W2381690723 title "Cloning and Sequence Analysis of TasA Gene from Mulberry Endophytic Bacteria Bacillus subtilis and Antibacterial Activity of its Expressed Product" @default.
- W2381690723 hasPublicationYear "2010" @default.
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