FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2381699797> ?p ?o ?g. }

• W2381699797 endingPage "S292" @default.
• W2381699797 startingPage "S291.6" @default.
• W2381699797 abstract "Purpose Penicillin-resistant invasive Streptococcus pneumoniae emerged in the 1990s and occurred subsequently with increasing frequency in Huntington, WV. We investigated the evolution of penicillin resistance during 23 years by tracking changes in the penicillin-binding protein gene 2b (pbp2b) among all invasive strains of capsular types 6, 9, 14, 19, and 23, the types likely to become resistant, to assess whether penicillin resistance evolved from other streptococci or was imported into the community. Methods We tested all consecutive invasive strains of S. pneumoniae isolated from children and adults admitted to hospital between 1983 and 2005 for penicillin susceptibility by E-test and capsular type by Quellung procedures. For genomic studies of the pbp2b gene, DNA was extracted from 289 strains, including 48 resistant (PRSP), 67 intermediate (PISP), 174 susceptible (PSSP), for restriction fragment length polymorphism (RFLP) using restriction enzyme HaeIII. A representative strain of each RFLP pattern was sequenced using internal primers and the assembled sequences were compared by multiple sequence alignment of DNA and amino acid sequences for homology. Results PSSP occurred every year and belonged to five RFLP patterns; 167 of 174 PSSP belonged to two RFLP patterns of nearly identical genomic sequences and identical amino acid sequences, remained stable throughout the study period, and were closely related to the reference strain R6. PRSP occurred almost every year starting in 1994, with the first one related to Spain 23F-1, belonged to five other RFLP patterns, and exhibited distinctly different genomic sequences from each other but were closely related to PRSP first identified in other countries. PISP occurred in all years except four and belonged to 15 RFLP patterns. By RFLP patterns, they all showed different genomic sequences from each other and from R6. The sequences of PISP reflected a spectrum between PSSP and PRSP. Conclusions These findings suggest that the PSSP persisted unchanged in the community throughout the study period. Unlike the PSSP, the PRSP appeared to have been imported into the community because all were closely related to PRSP previously identified in other countries, making it unlikely that antibiotic pressure in our community influenced the development of PRSP. However, it may have influenced the development of some PISP because only some PISP appear to have been imported based on a comparison of their genomic sequences with sequences registered in Genbank." @default.
• W2381699797 created "2016-06-24" @default.
• W2381699797 creator A5003031265 @default.
• W2381699797 creator A5030302464 @default.
• W2381699797 creator A5069911256 @default.
• W2381699797 date "2006-01-01" @default.
• W2381699797 modified "2023-09-27" @default.
• W2381699797 title "200 TRACKING EVOLVING PENICILLIN RESISTANCE OF STREPTOCOCCUS PNEUMONIAE THROUGH GENOMIC CHANGES IN THE PENICILLIN-BINDING PROTEIN GENE 2b." @default.
• W2381699797 doi "https://doi.org/10.2310/6650.2005.x0008.199" @default.
• W2381699797 hasPublicationYear "2006" @default.
• W2381699797 type Work @default.
• W2381699797 sameAs 2381699797 @default.
• W2381699797 citedByCount "0" @default.
• W2381699797 crossrefType "journal-article" @default.
• W2381699797 hasAuthorship W2381699797A5003031265 @default.
• W2381699797 hasAuthorship W2381699797A5030302464 @default.
• W2381699797 hasAuthorship W2381699797A5069911256 @default.
• W2381699797 hasConcept C104317684 @default.
• W2381699797 hasConcept C174802600 @default.
• W2381699797 hasConcept C2776653525 @default.
• W2381699797 hasConcept C2781253189 @default.
• W2381699797 hasConcept C501593827 @default.
• W2381699797 hasConcept C54355233 @default.
• W2381699797 hasConcept C86803240 @default.
• W2381699797 hasConcept C89423630 @default.
• W2381699797 hasConcept C94665300 @default.
• W2381699797 hasConceptScore W2381699797C104317684 @default.
• W2381699797 hasConceptScore W2381699797C174802600 @default.
• W2381699797 hasConceptScore W2381699797C2776653525 @default.
• W2381699797 hasConceptScore W2381699797C2781253189 @default.
• W2381699797 hasConceptScore W2381699797C501593827 @default.
• W2381699797 hasConceptScore W2381699797C54355233 @default.
• W2381699797 hasConceptScore W2381699797C86803240 @default.
• W2381699797 hasConceptScore W2381699797C89423630 @default.
• W2381699797 hasConceptScore W2381699797C94665300 @default.
• W2381699797 hasIssue "1" @default.
• W2381699797 hasLocation W23816997971 @default.
• W2381699797 hasOpenAccess W2381699797 @default.
• W2381699797 hasPrimaryLocation W23816997971 @default.
• W2381699797 hasRelatedWork W1892213375 @default.
• W2381699797 hasRelatedWork W1932616695 @default.
• W2381699797 hasRelatedWork W2026224862 @default.
• W2381699797 hasRelatedWork W2045128445 @default.
• W2381699797 hasRelatedWork W2063452348 @default.
• W2381699797 hasRelatedWork W2104240326 @default.
• W2381699797 hasRelatedWork W2137110239 @default.
• W2381699797 hasRelatedWork W2149759952 @default.
• W2381699797 hasRelatedWork W2152585568 @default.
• W2381699797 hasRelatedWork W2412692393 @default.
• W2381699797 hasVolume "54" @default.
• W2381699797 isParatext "false" @default.
• W2381699797 isRetracted "false" @default.
• W2381699797 magId "2381699797" @default.
• W2381699797 workType "article" @default.