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- W2381708138 abstract "Objective To construct artificial dermis on collagen chondrontin sulfate(CS) scaffold. Methods Collagen was compounded from CS and 1 ethyl 3 (13 dimethyl aminopropyl) carbodiimide(EDC) used as a crosslinker. Physical and chemical properties of the scaffold were characterized by electron spectroscopy for chemical analysis(ESCA), scanning electron micrograph(SEM), HE staining, and mechanical property test. Dermis fibroblasts were isolated from human embryo and were cultured on the scaffolds. Through histological testing, immunohistochemical testing and biochemical property testing, the property of collagen CS artificial dermis was compared with that of collagen spongy artificial dermis. Results Collagen CS had three dimension structure with porous. Compared with collagen scaffold, the mechanical property of collagen CS scaffold improved. There were more polar groups on the surface of collagen CS scaffold. The fibroblasts on the collagen CS scaffold grew well, and artificial dermis was constructed. Conclusion Collagen CS artificial dermis has more excellent biological and mechanical properties. Fibroblasts attach and proliferate better on collagen CS scaffold than on collagen scaffolds." @default.
- W2381708138 created "2016-06-24" @default.
- W2381708138 creator A5003103291 @default.
- W2381708138 date "2003-01-01" @default.
- W2381708138 modified "2023-09-25" @default.
- W2381708138 title "CONSTRUCTION OF ARTIFICIAL DERMIS ON COLLAGEN-CHONDRONTIN SULFATE SCAFFOLD CROSSLINKED BY 1-ETHYL-3-(13-DIMETHYL AMINOPROPYL) CARBODIIMIDE" @default.
- W2381708138 hasPublicationYear "2003" @default.
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