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- W2381710484 abstract "It was the first time to induce the callus of Carpesium macrocephalum Franch.et Sav by taking its root as the explants from its seeds in this paper.The results indicated that the asepsis germination rate of seed amounted to 98.80% when its seeds were sterilized with 70% alcohol for 5 min,then 50% NaClO_3 for 30 minutes.It also showed that such factors as basic media,types and concentration of hormones had a great effect on the induction rate,growth and browning time of the callus.The proper callus induction media were selected by orthogonal design,more-over,the kind and suitable level of hormone were ascertained.The basic media were the critical factors to callus induction,then NAA,the optimum medium for callus induction of Carpesium macrocephalum Franch.et Sav was B_5+NAA 3.0 mg/L+6-BA 0.2 mg/L.Moreover the best effects were obtained by using NAA of 2.0-3.0 mg/L+6-BA 0.2-0.4 mg/L in B_5 solid mediums.The induction rate of the callus was 100%,and the callus was primrose yellow and loose.By adding 500 mg/L V_C or 10 000 mg/L PVP,the callus kept primrose yellow and vigorous." @default.
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- W2381710484 date "2006-01-01" @default.
- W2381710484 modified "2023-09-25" @default.
- W2381710484 title "Study on the obtaining of sterile explants and callus induction of Carpesium macrocephalum Franch.et Sav" @default.
- W2381710484 hasPublicationYear "2006" @default.
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