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- W2381771223 abstract "Objective The aim of the study is to compare amplisensor quantitative PCR with RT-PCR (qualitative) in detecting HCV-RNA of different specimen and to evaluate the technique of amplisensor quantitative PCR. Methods Serum, urine and lymphocyte of 71 hemodialysis patients were tested for hepatitis C viral RNA by amplisensor quantitative PCR and RT-PCR (qualitative). Results Positive rate of HCV-RNA in seurm, lymphocyte, urine by amplisensor quantitative PCR (46.38%. 85.51%, 80.00%)was signdicanily higher than that by RT-PCR respectively (24.64%, 28.99%, 20.00%)(P0.05).The rate of simultaneously positive HCV-RNA in serum and lymphocyte was significantly higher by amplisensor quantitative PCR (39.13%) than by RT-PCR (8.70%) (P0.001). There was no relationship between the positive rate of HCV-RNA by RT- PCR and the quantilative level. Conclusion Compared with RT-PCR, amplisensor quantitative PCR technique has higher sen- sitivity in detecting HCV-RNA." @default.
- W2381771223 created "2016-06-24" @default.
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- W2381771223 date "2000-01-01" @default.
- W2381771223 modified "2023-09-24" @default.
- W2381771223 title "Study on amplisensor quantitative PCR and RT-PCR in detecting HCV-RNA" @default.
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