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- W2381811947 abstract "【Objective】The aims the experiment were to produce the polyclonal antibodies against toosendanin, and establish an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). 【Method】The haptens were synthesized by the reaction of toosendanin with succinate and glutarate, respectively, and characterized by HRMS, NMR and IR. Artificial immunogens and coating antigens were prepared separately with the mixed-anhydride method and the active ester method. Polyclonal antibodies were produced through immunizing rabbits. 【Result】Two haptens of toosendanin, toosendanin-succinil (TS) and toosendanin- glutaryl (TG), were synthesized successfully, and conjugated with BSA and OVA to form immunogens (TS-BSA and TG-BSA) and coating antigens (TS-OVA and TG-OVA). Two types of anti-toosendanin polyclonal antibodies (TS-Ab and TG-Ab) with high titers (6.4×104 and 3.2×105) were obtained. Optimized IC-ELISAs were established based on different antibody-antigen combinations. As measured with the combination of TS-Ab and TG-OVA, its limit of detection (LOD) for toosendanin was 0.03 μg·ml-1, IC50 was 1.52 μg·ml-1 and the linear range was from 0.01 μg·ml-1 to 100 μg·ml-1. While assayed by (TG-Ab, TG-OVA)combination, its LOD was 0.11 μg·mL-1 in the linear range of 0.1-100 μg·ml-1, and IC50 was 3.49 μg·ml-1. The recovery of toosendanin was 82.4%-106.0% in tap water sample based on TS-Ab and TG-OVA combination, and the precision (CV%) was 1.1%-14.3%.【Conclusion】 Anti-toosendanin polyclonal antibodies with high titers were obtained due to the reasonable design of haptens,and based on these antibodies, rapid and effective IC-ELISAs format for detecting toosendanin were established." @default.
- W2381811947 created "2016-06-24" @default.
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- W2381811947 date "2008-01-01" @default.
- W2381811947 modified "2023-09-27" @default.
- W2381811947 title "Haptens Design and Production of Antibodies Against Toosendanin" @default.
- W2381811947 hasPublicationYear "2008" @default.
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