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- W2381882724 abstract "Objective To investigate biological transport of norfloxacin by human periodontal ligament fibroblasts (HPDLF) to verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal.Methods HPDLF and MC3T3-E1 cells were incubated in norfloxacin solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by Bradford protein assay.Results The HPDLF intracellular contents were 0.095±0.032ng/μg, 0.096±0.052ng/μg, 0.104±0.078ng/μg at 1min, 5min, 10min respectively, incubated in 10μg/mL antibiotics solution. The MC3T3-E1 intracellular contents were 0.033±0.018ng/μg, 0.034±0.013ng/μg, 0.082±0.029ng/μg at 1min, 5min, 10min respectively, incubated in 10μg/mL antibiotics solution (P0.05). The MC3T3-E1 intracellular contents were 0.070±0.057ng/μg at 5min, incubated in 20μg/mL antibiotics solution.Conclusion HPLC is an accurate, sensitive method for measurement of the intracellular antibiotics. Norfloxacin can be transported by HPDLF. The transport is concentration-dependent, time-dependent and cell specific." @default.
- W2381882724 created "2016-06-24" @default.
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- W2381882724 date "2008-01-01" @default.
- W2381882724 modified "2023-09-24" @default.
- W2381882724 title "Biological transport of norfloxacin by human periodontal ligament fibroblasts" @default.
- W2381882724 hasPublicationYear "2008" @default.
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