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- W2381913209 abstract "Objective To test the expression of recombinant plasmid pcDNA-L1 and evaluate its efficiency to induce specific humoral immune responses. Methods The local HPV16-L1 gene was generated by polymerase chain reaction, inserted into pGEM-T Easy cloning vector, and then subcloned into the eukaryotic expression vector pcDNA3.1(-). The expression of recombinant plasmid pcDNA-L1, transferred in Cos-7 cells, was identified by immunofluorescence. The 6-week-old BALB/c mice were immunized via muscular 3 times with pcDNA-L1, thereafter the sera antibody of were measured by immunofluorescence and western-blot. Results With the immunofluorescence technique, L1 protein was found to express transiently in the transfected cells of the pcDNA-L1. Specific anti-HPV16-Ll antibodies could be detected by IFA at the end of 30,60 and 180 days after the first immunization, No specific antibodies could be detected in the control group of pcDNA3.1(-). Conclusions The recombinant plasmid pcDNA-L1 DNA constructed by us could induce specific immuno-humoral response in mice." @default.
- W2381913209 created "2016-06-24" @default.
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- W2381913209 date "2005-01-01" @default.
- W2381913209 modified "2023-09-24" @default.
- W2381913209 title "L1 Gene of Local Strain HPV16 Induces Immuno-humoral Response in Mice" @default.
- W2381913209 hasPublicationYear "2005" @default.
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