Matches in SemOpenAlex for { <https://semopenalex.org/work/W2381960003> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2381960003 abstract "In order to investigate the messenger ribonucleic acid(mRNA) and protein expression status of ANXA1 gene in nasopharyngeal carcinoma cell lines, so as to explore the correlation between ANXA1 methylation status and gene expression, four nasopharyngeal carcinoma(NPC) cell lines, including CNE1, CNE2, 5-8F, 6-10B, and immortalized non-neoplastic human nasopharyngeal epithelial cell line NP69 were cultured in vitro for research.Methylation status of ANXA1 gene was detected by methylation specific polymerase chain reaction(MSP), while mRNA expression level were also evaluated by reverse transcriptional polymerase chain reaction(RT-PCR).Subsequently, different end concentration(0 μmol/L as control, 0.1 μmol/L, 1 μmol/L, 5 μmol/L,10 μmol/L) of 5-aza-2′-deoxycytidine(5-aza-2dC) were added into culture medium of NPC cell lines for 72 hours′ de-methylation treatment, then methylation status of ANXA1 gene were detected by MSP and mRNA expression were evaluated by RT-PCR.In addition, ANXA1 protein expression was detected by Western-blotting.Without de-methylation treatment, ANXA1 gene was methylated in all the four NPC cell lines but NP69, and the methylation extent is correlated with differentiation state and metastasis potential of the cells.mRNA expression was lower in all of the four NPC cell lines without de-methylation treatment compared with NP69, and the expression level was correlated with gene methylation level.5-aza-2dC de-methylation treatment reversed ANXA1 methylation status, and increased the expression levels of mRNA and protein in all the four NPC cell lines.In summary, the current research verified down-regulated ANXA1 gene expression in NPC cell lines from both mRNA and protein level, the expression down-regulation was mainly caused by gene methylation, and 5-aza-2dC de-methylation treatment restored the down-regulated expression of ANXA1 in NPC cell lines to the level in the non-neoplastic cell line NP69." @default.
- W2381960003 created "2016-06-24" @default.
- W2381960003 creator A5019669403 @default.
- W2381960003 date "2009-01-01" @default.
- W2381960003 modified "2023-09-25" @default.
- W2381960003 title "DNA Methylation Inhibites ANXA1 Gene Expression in Nasopharyngeal Carcinoma Cell Lines" @default.
- W2381960003 hasPublicationYear "2009" @default.
- W2381960003 type Work @default.
- W2381960003 sameAs 2381960003 @default.
- W2381960003 citedByCount "0" @default.
- W2381960003 crossrefType "journal-article" @default.
- W2381960003 hasAuthorship W2381960003A5019669403 @default.
- W2381960003 hasConcept C104317684 @default.
- W2381960003 hasConcept C105580179 @default.
- W2381960003 hasConcept C126322002 @default.
- W2381960003 hasConcept C150194340 @default.
- W2381960003 hasConcept C153911025 @default.
- W2381960003 hasConcept C190727270 @default.
- W2381960003 hasConcept C2778997737 @default.
- W2381960003 hasConcept C33288867 @default.
- W2381960003 hasConcept C502942594 @default.
- W2381960003 hasConcept C509974204 @default.
- W2381960003 hasConcept C54355233 @default.
- W2381960003 hasConcept C55493867 @default.
- W2381960003 hasConcept C71924100 @default.
- W2381960003 hasConcept C81885089 @default.
- W2381960003 hasConcept C86803240 @default.
- W2381960003 hasConceptScore W2381960003C104317684 @default.
- W2381960003 hasConceptScore W2381960003C105580179 @default.
- W2381960003 hasConceptScore W2381960003C126322002 @default.
- W2381960003 hasConceptScore W2381960003C150194340 @default.
- W2381960003 hasConceptScore W2381960003C153911025 @default.
- W2381960003 hasConceptScore W2381960003C190727270 @default.
- W2381960003 hasConceptScore W2381960003C2778997737 @default.
- W2381960003 hasConceptScore W2381960003C33288867 @default.
- W2381960003 hasConceptScore W2381960003C502942594 @default.
- W2381960003 hasConceptScore W2381960003C509974204 @default.
- W2381960003 hasConceptScore W2381960003C54355233 @default.
- W2381960003 hasConceptScore W2381960003C55493867 @default.
- W2381960003 hasConceptScore W2381960003C71924100 @default.
- W2381960003 hasConceptScore W2381960003C81885089 @default.
- W2381960003 hasConceptScore W2381960003C86803240 @default.
- W2381960003 hasLocation W23819600031 @default.
- W2381960003 hasOpenAccess W2381960003 @default.
- W2381960003 hasPrimaryLocation W23819600031 @default.
- W2381960003 hasRelatedWork W2023662256 @default.
- W2381960003 hasRelatedWork W2092688423 @default.
- W2381960003 hasRelatedWork W2162792820 @default.
- W2381960003 hasRelatedWork W2347847199 @default.
- W2381960003 hasRelatedWork W2353817712 @default.
- W2381960003 hasRelatedWork W2360280952 @default.
- W2381960003 hasRelatedWork W2363714510 @default.
- W2381960003 hasRelatedWork W2364479539 @default.
- W2381960003 hasRelatedWork W2370650230 @default.
- W2381960003 hasRelatedWork W2373554668 @default.
- W2381960003 hasRelatedWork W2380600304 @default.
- W2381960003 hasRelatedWork W2383104420 @default.
- W2381960003 hasRelatedWork W2383710130 @default.
- W2381960003 hasRelatedWork W2390057544 @default.
- W2381960003 hasRelatedWork W2390288881 @default.
- W2381960003 hasRelatedWork W2390986524 @default.
- W2381960003 hasRelatedWork W2414805490 @default.
- W2381960003 hasRelatedWork W2552609903 @default.
- W2381960003 hasRelatedWork W3141289787 @default.
- W2381960003 hasRelatedWork W324811617 @default.
- W2381960003 isParatext "false" @default.
- W2381960003 isRetracted "false" @default.
- W2381960003 magId "2381960003" @default.
- W2381960003 workType "article" @default.