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- W2381961490 abstract "To increase the expression yield of Gloshedobin by Pichia pastrois,the recombinant thrombin-like enzyme was expressed ith 500ml shaking flask.By studying the concentration and activity of secreted protein under different conditions:components of medium,pH,and the time of fermentation,the optimal fermentation conditions were obtained:rich medium is more beneficial for secretion expression than minimal medium,the optimal pH 6.0, and the optimal fermentation time 36 hours.The amidolytic actvity can be detected from the supernatant,and the expression level of target protein is about 4?mg/L." @default.
- W2381961490 created "2016-06-24" @default.
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- W2381961490 date "2003-01-01" @default.
- W2381961490 modified "2023-09-22" @default.
- W2381961490 title "Optimization of Fermentation Conditions for Express of Gloshedobin in Yeast Pichia Pastoris" @default.
- W2381961490 hasPublicationYear "2003" @default.
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