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- W2381970673 abstract "Using stem tips of two verbena cultivars as explants,the tissue culture and rapid propagation system of modern verbena cultivars was established on the base of screening suitable disinfection method,and proper culture mediums for regeneration.The results indicated that the most effective disinfection method was to soak explants with 70% alcohol for 15 s and 0.1% HgCl2 for 4 min,in which the lower contamination rate reached to 10% and maximum surviving rate to 87.5%.Concerning the different hormone concentration requirements of induction and subculture,obvious genotype difference could be observed.The optical subculture medium for big flower Quartz and small flower Aztec was MS+BA 1.0 mg/L+NAA 0.2 mg/L and MS+BA0.5 mg/L+NAA0.1 mg/L respectively.For a given cultivar,high concentration of 6-BA might induce calluses from leaf directly,at the same time,a few adventitious buds were gained from the calluses.Root of both cultivars was easily induced on 1/2MS medium supplemented with 0.05 mg/L NAA,and rooting rate of 96%~98% could be obtained." @default.
- W2381970673 created "2016-06-24" @default.
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- W2381970673 date "2010-01-01" @default.
- W2381970673 modified "2023-09-25" @default.
- W2381970673 title "Tissue Culture and Rapid Propagation of Verbena (Verbena hybrida)" @default.
- W2381970673 hasPublicationYear "2010" @default.
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