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- W238201244 abstract "A series of isonuclear cytoplasmic petite (ϱ−) mutants presenting various patterns of large deletions and retentions in the C321R — E514R region was used to study the functional organization of mitochondrial DNA. In vivo transcription products of ϱ− mitochondria were analysed by gel electrophoresis and, in an attempt to locate the 23 S ribosomal RNA gene in the mitochondrial genome, by RNA-DNA hybridization. The clones carrying the CR and EP genetic markers contained the 23S mitochondrial rRNA gene and transcribed it in vivo. The clones keeping only the ER marker appeared to have at least a partial sequence of the 23 S rRNA gene; in one clone the whole 23 S rRNA sequence was still present and correctly transcribed. The clones keeping only the CR marker region also had a part of the region sequence complementary to 23 S RNA. No ϱ− clone, in this series, possessed a DNA sequence corresponding to the 16 S rRNA. The possibility that the drug-resistance mutation E514R might be in the 23 S rRNA gene is discussed." @default.
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- W238201244 date "1974-09-01" @default.
- W238201244 modified "2023-09-27" @default.
- W238201244 title "Physical and genetic organization of petite and grande yeast mitochondrial DNA" @default.
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- W238201244 doi "https://doi.org/10.1016/0022-2836(74)90304-0" @default.
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