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- W2382014042 abstract "A pair of primers for the 49 ku es gene of Trichinella spiralis were designed according to the published sequence in GenBank (accession number M64242).The gene encoding 49 ku ES antigen were specifically amplified by RT-PCR.The target gene was directly cloned into the pMD18-T Vector,and then transformed into Escherichia coli strain TG1.The recombinant plasmids were identified by enzymatic digestion with EcoR I and BamH I and PCR amplification.The positive plasmids were subjected to sequencing.The 49 ku es antigen genes in six Trichinella isolates of pre-encysted larvae were cloned successfully.Sequence analysis indicated that the 49ku es gene was 948bp in length and was conserved,and the homology rates of the sequences were quite high among different Trichinella isolates." @default.
- W2382014042 created "2016-06-24" @default.
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- W2382014042 date "2007-01-01" @default.
- W2382014042 modified "2023-09-23" @default.
- W2382014042 title "Cloning and sequence analysis of a 49 ku es antigen gene of different trichinella isolate pre-encysted larvae" @default.
- W2382014042 hasPublicationYear "2007" @default.
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