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- W2382024634 abstract "Objective: To explore a method to obtain a large number of more mature and homogenous cell types from ESCs.Methods: A novel protocol was developed by combining ascorbic acid and co-cultured inducing strategies to control the differentiation of embryonic stem cells(ESCs),and then evaluated the structural and functional properties of these ESC-derived CMs(ESCMs) by im-munohistochemistry,flow cytometric analysis,RT-PCR,and transmission electron microscopy.Results: The majority(average,90.1± 3.40) % of ESCs-derived aggregates(embryoid bodies,EBs) in the co-culture systems exhibited spontaneous contractions,as ESCs cul-tured alone,or only with ascorbic acid,the average percentage of beating aggregates was(20.1±3.82) % and(29.6±6.03) %.Both the number of beating EBs and the percentage of functional CMs were increased significantly in mimic cardiac microenvironment.The auto-maticity of beating also maintained for more time and cellular morphology of beating cells at late stage was similar to native CMs.Con-clusion: The cardiac microenvironment is the key inducer in directing the site-specific differentiation of ESCs.Ascorbic acid and co-cul-tured inducing strategies can make ESCs directionally differentiate into Cardiomyocytes(CMs)." @default.
- W2382024634 created "2016-06-24" @default.
- W2382024634 creator A5042628509 @default.
- W2382024634 date "2009-01-01" @default.
- W2382024634 modified "2023-09-23" @default.
- W2382024634 title "Differentiation of Embryonic Stem Cells towards Cardiomyocytes in Mimic Cardiac Microenvironment in vitro" @default.
- W2382024634 hasPublicationYear "2009" @default.
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