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- W2382056135 abstract "To express very virulent infectious bursal disease virus(vvIBDV) strain LX VP2 protein by Drosophila S2 cell expression system and to further analyze the antigenicity of the recombinant protein,the VP2 gene from vvIBDV strain LX was amplified by reverse transcription(RT)-PCR and cloned into Drosophila S2 cell expression vector pMT/BiP/V5/HisA to obtain a recombinant expression vector pMT/BiP/V5/HisA-VP2. The plasmid from the pMT/BiP/V5/HisA-VP2 vector and screening plasmid pCoBlast were cotransfected into S2 cells to generate VP2 fusion protein. Then the expressed protein was purified and its antigenicity was analyzed. Results show that a specific and simple band with a size of about 49 kDa was observed as examined by Westem Blot using a monoclonal antibody raised against VP2 protein indicating that IBDV VP2 protein can be effectively expressed in S2 cells followed by secreting into the supernatant with a good antigenicity,and it can used as an antigen for diagnosis." @default.
- W2382056135 created "2016-06-24" @default.
- W2382056135 creator A5070831240 @default.
- W2382056135 date "2014-01-01" @default.
- W2382056135 modified "2023-09-23" @default.
- W2382056135 title "Expression of Infectious Bursal Disease Virus VP2 Protein in Drosophila S2 Cells and Its Antigenicity Analysis" @default.
- W2382056135 hasPublicationYear "2014" @default.
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