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• W2382090687 abstract "Rachycentron canadum is a species widely distributed in warm waters of the bottom of the Atlantic,Indian and western Pacific. It is also a kind of important economic fish. For a long time,affected by natural and human factors,the number of wild Rachycentron canadum has decreased sharply. In order to provide valuable and referable data for the making of Rachycentron canadum conservation policy and for management of the shrinking population,researches on genetic diversity needs to be started.In this study,883 bp mtDNA cytochrome b sequences from 34 individuals collected from Lianyungang( LYG,n =11),Zhoushan( ZS,n =8),Fangcheng( FC,n =15) were sequenced. Results were as follows:1. Genetic diversity: Five variable sites detected in aligned sequences defined six haplotypes,the average haplotypes( h) and nucleotide diversity( π) were 0. 324 and 0. 0004 respectively,indicating a low genetic diversity. LYG contained the highest values of genetic diversity,the haplotype and nucleotide diversity were0. 473 ± 0. 162 and 0. 00057 ± 0. 00593 respectively,whereas ZS showed no genetic variation. 2. Genetic differentiation: Pairwise FSTfrom LYG to ZS and FC were 0. 029( P = 0. 00) and 0. 042( P = 0. 00)respectively,and FSTbetween ZS and FC was- 0. 048 03( P = 0. 00),indicating that there was only relatively low differentiation between LYG and the other two populations,and no obvious differentiation was found in ZS and FC. Analysis of Molecular Variance( AMOVA) showed that most of genetic variation in three populations distributed within populations. This may be due to the fact that the East China Sea,South China Sea and the Yellow Sea have continuity,which promotes groups of communication between genes. Chinese offore Rachycentron canadum showed low genetic diversity( Hd = 0. 324 ± 0. 103,Pi = 0. 0004),and FST,AMOVA and TCS network showed no genetic variation and no obvious geographical pattern system within populations. 3. Population expanding: The haplotype network was star-like,Tajima's D and Fu's Fs values in three populations of combined neutrality tests were significantly negative( FST=- 1. 92240,P ﹤ 0. 00; FST=- 5. 735,P ﹤ 0. 00),indicating that cobia in coastal waters of China had experienced populations expansion in history. According to observed τ value( 0. 364),the deduced population expansion of Cobia occurred about 3. 1- 1. 2 million years ago,in last glacial of the maximum. 4. We can speculate that these three groups maybe belong to the same population,for their geographic genetic differention was not obvious. It should be noted that these results do not exclude the possibility of difference in other regions for the number of geographic populations and samples is small. In order to understand and assess the genetic diversity of Rachycentron canadum,we need to use AFLP fingerprinting,microsatellite DNA and relevant techniques,which can understand the genetic differentiation of Rachycentron canadum and provide a theoretical basis for the protection of wild germplasm." @default.
• W2382090687 created "2016-06-24" @default.
• W2382090687 creator A5048647316 @default.
• W2382090687 date "2014-01-01" @default.
• W2382090687 modified "2023-09-25" @default.
• W2382090687 title "On mtDNA cytochrome b gene sequence variation of Cobia( Rachycentron canadum)" @default.
• W2382090687 hasPublicationYear "2014" @default.
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