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- W2382116703 abstract "Aim To compare different show of the muscle fibre types after acid and alkaline pre-incubations of the myosin-ATPase method.Methods Cryostate sections of rat soleus muscle were stained with the myosin adenosine triphosphatase (mATP) method. Results ①After pre-incubation of sections at pH4.3, 4.6 and pH9.4, 10.4, based on the pattern of enzyme reactivity, the fibres can be classified in type Ⅰ, Ⅱ (subtypes A, AB, and B) and type Ⅰ, Ⅱ fibres, respectively. Furthermore, at pH4.3 pre-incubation the result of classification of the soleus fibres is much better than that of at pH4.6. The type Ⅱ fibres have more subtypes and lower ratio at acid condition than that at alkaline condition. ②Pre-incubation and staining solution were both developed toward acid condition with time passed.Conclusion The myosin-ATPase method can be utilized in the classification of extrafusal fiber in rat soleus muscle, but the pH lability of this method should be taken into account." @default.
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- W2382116703 date "2005-01-01" @default.
- W2382116703 modified "2023-09-23" @default.
- W2382116703 title "Effects of the histochemical mATPase reaction at different pH values on the classification of extrafusal fiber in rat soleus muscle" @default.
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