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- W2382123092 abstract "Objective To express the core protein (Gag) of HIV-1 subtype C and lay a foundation of diagnosis of HIV infection and development of HIV vaccine.Methods The Gag gene fragment of HIV-1 was amplified by PCR,inserted downstream to the tac promoter of prokaryotic expression vector pGEX-5X-1.The constructed recombinant plasmid was transformed to E.coli BL21(DE3) for expression under induction of IPTG.The expressed product was identified by SDS-PAGE and Western blot.Results The GST fusion protein,with a relative molecular weight of 82 000,was expressed in E.coli and recognized by McAb to p24.Conclusion Recombinant Gag protein was effectively expressed in E.coli and showed good antigenicity.The expressed protein might be used for the detection of HIV infection and the study on immunization in animals." @default.
- W2382123092 created "2016-06-24" @default.
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- W2382123092 date "2005-01-01" @default.
- W2382123092 modified "2023-09-28" @default.
- W2382123092 title "Expression of Gag Protein of HIV-1 Subtype C in E.coli" @default.
- W2382123092 hasPublicationYear "2005" @default.
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