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- W2382126023 abstract "Objective To establish an experimental model of HCV C-HBV X coexpression protein and to explore its effect on insulin-like growth factor 1 receptor(IGF-1R).Methods The HBV X gene was recoverd by enzyme excision,and cloned into PBK-CMV and PBK-HCV C for getting the recombine plasmids PBK-X and PBK-X-C.The plasmids PBK-CMV,PBK-X,PBK-HCV C and PBK-X-C were transfected into HepG2 cells with liposome.After selected with G418,resistant colonies were obtained.The reverse transcription PCR and Western blot was analyzed to show HBV X and HCV core protein expressed.IGF-1R were analyzed by reverse transcription PCR and Western blot.Results The recombinant plasmid PBK-X-C could express HBV X and HCV core protein efficiently under the control of vector's promoter.IGF-1R of the cells coexpressed HCV C-HBV X protein was higher than that of the cells expessed HBV X,HCV C and vector along.Conclusion HBV X-HCV C coexpression protein can increase the expression of IGF-1R in HepG2 cells.It suggests that HBV and HCV can cooperate with carcinogenesis." @default.
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- W2382126023 date "2007-01-01" @default.
- W2382126023 modified "2023-09-23" @default.
- W2382126023 title "Effect of hepatitis B virus X gene and hepatitis C virus C gene coexpression protein on IGF-1R of HepG2 cells" @default.
- W2382126023 hasPublicationYear "2007" @default.
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