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- W2382133060 abstract "ObjectiveTo synthesize genes of the heavy chain variable region(VH) and light chain variable region(VL) of single-chain antibody of human cardiac myosin heavy chain for the development of myocardial imaging agents: single-chain antibody of human cardiac myosin heavy chain.MethodsTo extracted total RNA of the anti-HCMHC McAb hybridoma using TRIZOL reagent,synthesize the first-strand cDNA,using this first-strand cDNA as template,with specific primers,DNA polymerase and four single nucleotides,amplifiy the genes of the heavy chain variable region(VH) and light chain variable region(VL) by PCR.To identifiy products of each step and study relationship between RNA stability and storage temperature and optimize cycle selection temperature with MgCl2 concentration.ResultsThe purity of the first-strand cDNA reached 95%,PCR products by agarose gel electrophoresis showed a single band with a bright swimming,molecular weight of VH and VL genes were 340bp and 320bp,which was consistent with literature reports.ConclusionSynthesized genes of VH and VL,laid the foundation for the development of myocardial imaging agents: single-chain antibody of human cardiac myosin heavy chain." @default.
- W2382133060 created "2016-06-24" @default.
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- W2382133060 date "2012-01-01" @default.
- W2382133060 modified "2023-09-23" @default.
- W2382133060 title "Synthesiaed Genes of VH and VL of Single-Chain Antibody of Human Cardiac Myosin Heavy Chain" @default.
- W2382133060 hasPublicationYear "2012" @default.
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