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- W2382146112 abstract "Objective:To explore the effect of transvaginal ultrasonic irradiation on apoptosis of human embryo villous trophoblast and the reversibility,explore the effects of Bcl-2 protein and activated Caspase-3 protein on apoptosis of trophoblast.Methods:A total of 50 patients were selected and randomly divided into five groups,10 patients in each group.The patients in four experimental groups were irradiated by transvaginal ultrasound for 10 minutes,then villous samples were collected after artificial abortion,the other group was control group.TUNEL method was used to detect the apoptosis indexes of villous trophoblast in the five groups.Immunohistochemical method was used to detect the expression levels of Bcl-2 protein and activated Caspase-3 protein in villous trophoblast.Results:Transvaginal ultrasonic irradiation had impact on apoptosis of villous trophoblast.After transvaginal ultrasonic irradiation for 10 minutes,the apoptosis index of human embryo villous trophoblast on the first day was the highest,the expression level of Bcl-2 protein was the lowest,the expression level of Caspase-3 protein was the highest;with the extension of embryo-collecting time,the apoptosis index decreased,the expression level of Bcl-2 protein increased gradually,the expression level of Caspase-3 protein decreased gradually;there was no statistically significant difference in the apoptosis index,the expression level of Bcl-2 protein,and the expression level of Caspase-3 protein on the fifth day,the third day,and the fifth day respectively after irradiation between experimental groups and control group(P0.05).Conclusion:Transvaginal ultrasonic irradiation for 10 minutes can induce increase of apoptosis of human embryo villous trophoblast,but the change showed short term reversibility,Bcl-2 protein and activated Caspase-3 protein play important roles in apoptosis of trophoblast." @default.
- W2382146112 created "2016-06-24" @default.
- W2382146112 creator A5082894352 @default.
- W2382146112 date "2012-01-01" @default.
- W2382146112 modified "2023-09-23" @default.
- W2382146112 title "Study on apoptosis of human embryo villous trophoblast,Bcl-2 protein,and Caspase-3 protein after transvaginal ultrasonic irradiation" @default.
- W2382146112 hasPublicationYear "2012" @default.
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