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- W2382164052 abstract "Objective:To investigate the role of DMSO in growth and apoptosis of human umbilical vascular endothelial cells(HUVEC).Methods:Light and fluorescence microscope and flow cytometry(FCM)were used to determine morphological changes,cell cycle distribution and apoptosis rate of vascular endothelial cells.Results:The cells with DMSO and bovine serum and FGF became elongated and the nuclei were intact.The cells in G 2/M phase increased and the CM(139.7mmcells in S phase decreased.There was no difference in apoptosis rate between the normal and)the DMSO treated group.When the cells were deprived of bovine serum and FGF,they started to die and disintegrated into apoptotic bodies and the nuclei condensed and fragmented.But when the cells were added DMSO at the same time,they attached to the dishes and became spreading and elongated,and the nuclei were intact,just like those of normal cells.The cells in S phase increased and the cells in G 2/M phase decreased significantly.The apoptosis rate approached that of the normal group.Conclusion:DMSO inhibits the apoptosis of VEC induced by deprivation of bovine serum and FGF and has no significant effect on the apoptosis of VEC with bovine serum and FGF.The data indicates that DMSO might affect the growth and apoptosis of VEC by regulating cell cycle and promoting cell spreading." @default.
- W2382164052 created "2016-06-24" @default.
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- W2382164052 date "2003-01-01" @default.
- W2382164052 modified "2023-09-26" @default.
- W2382164052 title "Effect of DMSO on the growth and apoptosis of human vascular endothelial cells" @default.
- W2382164052 hasPublicationYear "2003" @default.
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