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- W2382189096 abstract "AIM To investigate the expression of angiotensinogen (Ang) mRNA and the changes of angiotensin Ⅱ(AngⅡ) contents in rat hypothalamus during stress. METHODS Forty eight male rats were randomly allocated to three groups. Swimming rats were treated with swimming stimulation in 4℃~6℃ cold water; electric shock rats were treated with repetitive 0.5~2 Hz, 60~100 V electric shock on foot for 1wk,twice a day for 2 h each time; and control rats weren't treated with any stimulation. Then Ang mRNA expression in hypothalamus was determined by using in situ hybridization technique, and AngⅡ contents were detected by RIA. RESULTS The area percentage and integral absorbance of Ang mRNA positive expression were significantly increased [swimming group (2.48±0.40)% and (0.75± 0.09 ), electric shock group (2.94±0.82)% and (1.83± 0.41 ), vs control group (0.20±0.04)% and (0.24±0.12), P 0.01], AngⅡ contents were also significantly increased [swimming group (141±67) ng·g -1 , electric shock group (84±25) ng·g -1 , vs control group (56±16) ng·g -1 , P 0.01 , P 0.05], and the increased rates of Ang mRNA [(1216±196)% and (1441±402)%] surpassed the ones of AngⅡ contents [(295±118)% and (149±44)%] ( P 0.01 ). CONCLUSION The local renin angiotensin system (RAS) in hypothalamus is obviously activated during stress, which strongly supports the view that local RAS contributes to stress reaction." @default.
- W2382189096 created "2016-06-24" @default.
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- W2382189096 date "2002-01-01" @default.
- W2382189096 modified "2023-09-27" @default.
- W2382189096 title "Expression of angiotensinogen mRNA in rat hypothalamus during stress" @default.
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