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- W2382202112 abstract "The protein kinases were identified at different developmental stages of apple (Malus domestica L. Borth. cv. Red Starking) and grape (Vitis vinifera L. × V.lubrusca L. cv. Kyoho) fruit, and these protein kinases were found to be greatly activated by Ca 2+ . This demonstrated that calcium_dependent protein kinase plays an important role in apple and grape fruits. The results also showed that calmodulin (CaM) had no stimulation effect, but the CaM antagonists, such as calmidazolium, W7 and trifluoroacetic acid (TFA), had some inhibitory effects on the activities of this kind of protein kinase in both fruits, indicating that the detected protein kinases may be a kind of calmodulin_like domain protein kinase or calcium_dependent protein kinase (CDPK). There were distinct differences between apple and grape fruits, both in the characteristics and in the activity evolution patterns of the protein kinases during fruit development. The activity of calcium_dependent protein kinase was little changed in the three stages of apple fruit development, while in grape fruit the activity of this protein kinase was much higher in stage Ⅱ than those in stage Ⅰ and stage Ⅲ. The calcium_dependent protein kinase in apple fruits was strongly activated by Mn 2+ , and it was sensitive to heat treatment, but in grape fruits it was neither stimulated by Mn 2+ nor sensitive to high temperature. Besides, phosphatidyl_serine (PS) had little effect on the activities of calcium_dependent protein kinase in grape fruits, but had stimulative effects in apple fruits. It was thus proposed that there might be a kind of calcium_ and phosphatide_activated protein kinase, i.e., PKC, in apple fruits. The differences among the kinds, characteristics and the activity evolution patterns of protein kinases during apple and grape fruit development, suggest that the protein kinases may be involved in the process of fruit development." @default.
- W2382202112 created "2016-06-24" @default.
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- W2382202112 date "2000-01-01" @default.
- W2382202112 modified "2023-09-25" @default.
- W2382202112 title "Characterization of Protein Kinases in Apple and Grape During Fruit Development" @default.
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