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- W2382204471 abstract "The immobilization procedures of soybean lipoxygenase(LOX) using atlapulgite or talc as carrier are optimized and scaled up with a ratio of 897 U/mg atlapulgite stirring 30 min in a 0.05 mol/L and pH 7.0 phosphate buffer solution at 20 ℃,and a ratio of 238 U/mg talc stirring 15 min in a 0.05 mol/L and pH 8.0 phosphate buffer solution at 10 ℃.When kept at 0～4 ℃ for 20 days the immobilized enzyme loses activity by 19.2% for atlapulgite or 17.7% for talc.The immobilized enzyme leads a 9.6% higher yield for atlapulgite or 42.5% higher yield for talc in enzymatic reaction than the free LOX.The immobilized LOX beads using sodium alginate as carrier are much more stable than the free one when kept at a higher temperature,in broader pH range and in organic solvents.After being kept in glycerol〔φ(glycerol)=75%〕 for 33 days,the beads barely lose activity.The beads still maintain 90% activity at 50 ℃ for 60 min." @default.
- W2382204471 created "2016-06-24" @default.
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- W2382204471 date "2004-01-01" @default.
- W2382204471 modified "2023-10-11" @default.
- W2382204471 title "Immobilization of Soybean Lipoxygenase and Enhancement of Stability" @default.
- W2382204471 hasPublicationYear "2004" @default.
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