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- W2382204927 abstract "Oxidative damage characteristic of Herring Sperm DNA by hydroxyl radical(·OH) which was excited by TiO2 under UV irradiation(200-275 nm,66.4 Lx) was studied.The process of DNA damage was detected by gel electrophoresis and high performance liquid chromatographic analysis.Electron spin resonance(ESR) and spectrophotometry were used to determine the oxygen species and the relative concentration of H2O2,respectively.The mechanism of DNA damage was studied using 8-hydroxy-2′-deoxyguanosine(8-OHdG) as an internal standard.The results showed that DNA can be damaged by ·OH generated from photocatalysis.Compared with UV or Dark/TiO2 system,the degree of DNA damage reached the maximum in the UV/TiO2 system under the condition of DNA concentration 10 mg/L,TiO2 concentration 1.5 g/L and pH 7-8.The damage was caused by the process of ·OH oxidation of TiO2 under UV irradiation and deep mineralization of DNA took place.Guanine was easy to damage than the other groups in DNA.8-OHdG was one intermediate product of DNA damage and a special product of guanine damage." @default.
- W2382204927 created "2016-06-24" @default.
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- W2382204927 date "2011-01-01" @default.
- W2382204927 modified "2023-09-25" @default.
- W2382204927 title "Damage of Deoxyribonucleic Acid Caused by Hydroxyl Radical" @default.
- W2382204927 hasPublicationYear "2011" @default.
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