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- W2382206582 abstract "Objective To investigate the expression of endogenous α-crystallin in retinal ganglion cells(RGCs) in rats in vitro.Methods RGCs of rats were primary cultured.The cells right after digested with trypsogen and the cells primary cultured from 1 day to 7 days were observed by immunofluorescence staining and confocal laser scanning microscopy,the expression of endogenous α-crystallin in the RGCs with primary culture from 1 day to 7 days,9 days,12 days were detected by RT-PCR.Results Immunofluorescence staining showed that,the positive staining of endogenous α-crystallin was observed in little RGCs membrane at 2 days after primary culture,and reached the peak from 3 days to 5 days after primary culture,which in ring shape,then gradually decreased.The results of mRNA detection showed that,the expression of endogenous α-crystallin were observed in the whole procession of primary culture,reached the peak from 3 days to 5 days,then gradually decreased.Conclusion The endogenous α-crystallin can be expressed in the RGCs of rats with temporary increase,which may be relative to the survival and proliferation of RGCs." @default.
- W2382206582 created "2016-06-24" @default.
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- W2382206582 date "2010-01-01" @default.
- W2382206582 modified "2023-09-23" @default.
- W2382206582 title "Expression of endogenous α-crystallin in retinal ganglion cells of rats and its change rule" @default.
- W2382206582 hasPublicationYear "2010" @default.
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