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- W2382265171 abstract "【Objective】 This study aimed to screen Streptoveticillium mobaraense mutants with high-yield of microbial transglutaminase(MTG)using cellulose acetate film(CAF)and 96-well microtiter plate based high-throughput screening(HTS)methods.【Method】 CAF and 96-well microtiter plate were applied in high-throughput primary screening of UV treated S.mobaraense.High-yield candidates were further confirmed by stout tube fermentation and shaking flask method.Conditions of 96-well microtiter plate cultivation were optimized.【Result】 1 strain with high-yield of MTG(MTG activity as 4.9 U/mL)was selected by CAF assay from a library of over 3 000 mutants with the enzyme activity improved by 80% compared to the parent strain(MTG activity as 2.7 U/mL).Optimized conditions of 96-well microtiter plate fermentation were as follows:colonies with a diameter of 3 mm were inoculated to 150 μL/well fermentation medium before being cultivated at 30 ℃ 200 r/min for 72 h for enzyme assay.2 strains were screened from 3 415 mutants with MTG activities of 5.4 U/mL,80% higher than and starting strain with MTG activity of 3.0 U/mL.【Conclusion】 1 and 2 high-yield strains were selected using CAF and 96-well microtiter plate,respectively.Both methods developed in this research provided efficient HTS approaches at a low cost in short period." @default.
- W2382265171 created "2016-06-24" @default.
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- W2382265171 date "2013-01-01" @default.
- W2382265171 modified "2023-09-23" @default.
- W2382265171 title "Novel high-throughput screening of Streptoverticillium mobaraense with high microbial transglutaminase yield" @default.
- W2382265171 hasPublicationYear "2013" @default.
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