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- W2382275134 abstract "Objective:To estabilish a solid phase extraction and high performance liquid chromatography(SPE-HPLC)method for determination of methotrexate in human milk of placenta implantation women.Methods:Milk samples were prepared with C18solid phase extraction column.The chromatographic separation was performed on a Agilent ZOBAX SB-C18(4.6 mm×250 mm,5 μm) column.The mobile phase consisted of 0.05% acetic acid-acetonitrile(88∶ 22),flow rate was 1.0 mL·min-1.The detection wavelength was 306 nm.Results:The linear range for methotrexate was 0.5-5 μg·mL-1.The average recoveries of high,middle and low three concentration were 99.8%,100.9% and 99.1%,RSD were both less than 10%.The intra-day and inter-day RSD were less than 5.8%.Conclusion:The method is accurate,sensitive and simple,and suitable for the determination of methotrexate concentration in human milk." @default.
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- W2382275134 date "2011-01-01" @default.
- W2382275134 modified "2023-09-24" @default.
- W2382275134 title "SPE-HPLC determination of methotrexate concentration in human milk" @default.
- W2382275134 hasPublicationYear "2011" @default.
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