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- W2382359771 abstract "In order to get alpha-interferon(INF-α) with high level secretive expression and high antiviral activities,the porcine alpha-interferon gene was amplified by PCR,and a DNA fragment of 501 bp,the open reading framework(ORF) was obtained.Then,the target gene and pPICZαC were digested with EcoRI/XbaI and were linked.The recombinant plasmid of pPICZαC-IFN was linearized by SacI and electroporated into Pichia pastoris X-33.PCR assay was used to identify colonies.The high copy recombinat strains were screened and induced by regulation of methanol utilization.IFN-α protein was detected by SDS-PAGE and Western-blotting analysis.The result showed the IFN-α protein with a raletive molecular mass of 19 400 was expressed in Pichia pastoris X-33.The antiviral activity of IFN-α against vesicular stomatitis virus(VSV) was investigated on the Vero cell and the result indicated that IFN-α could inhibit VSV and the activity was 4.04×106 IU/L." @default.
- W2382359771 created "2016-06-24" @default.
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- W2382359771 date "2010-01-01" @default.
- W2382359771 modified "2023-09-23" @default.
- W2382359771 title "The Secretive Expression of Porcine Alpha-Interferon Gene in Pichia pastoris" @default.
- W2382359771 hasPublicationYear "2010" @default.
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