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- W2382409006 abstract "Objective Our aims to establish the three dimensional culture model of lung cancer cell line A549 and to study the cellular behaviors of these cells.Methods Transmission Electron Microscopy results demonstrated the nanofiber networks formed by the Matrigel hydrogel.The morphology,distribution and viability of A549 cells in three dimensional culture were identified by fluorescence microscopy.MTT assay was used to assess 5Fluorouracil cytotoxicity to the cell colonies.The adhesion ability of cells of three dimensional culture and twodimensional culture was measured by their adhesion percentage to ECM proteins.Results Matrigel formed nanofiber networks and could be used as culture matrix to mimic the cell growth conditions in vivo and the three dimensional culture for cells in vitro. In this study,cells grown in Matrigel formed multicellular colonies and good viability and biocompatibility with the matrix. The cell resistance to 5 Fluorouracil increased when cells formed multicelluar colonies following the culture time and reduced the sensibility of cells to the drug.The adhesion assay indicated that there were significant differences of cells in three dimensional culture to ECM proteins compared to two dimensional culture.Conclusion Our present study showed that three dimensional culture of A549 cells in matrigel matrix influenced the cellular behaviors including the cell growth,morphology,viability,drug resistance alteration and the adhesion ability which were significantly different from cells in two dimensional culture.Three dimensional culture can provide significant research basis for the study of drug resistance and the cell signaling pathways in the future." @default.
- W2382409006 created "2016-06-24" @default.
- W2382409006 creator A5043366101 @default.
- W2382409006 date "2009-01-01" @default.
- W2382409006 modified "2023-09-25" @default.
- W2382409006 title "Three dimensional culture model of lung cancer cell line A549 and the study of their cellular behaviors" @default.
- W2382409006 hasPublicationYear "2009" @default.
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