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- W2382431656 abstract "Fertilized embryo sacs of Nicotiana tabacum cultivar G - 80 and N. tabacum var. macrophylla were isolated by enzymatic maceration and grinding, then the zygotes or two-celled proembryos were isolated by microdis-section. Three to five zygotes or two-celled proembryos were embedded in agnrose in mi-crochambers (Millicell-CM, Millipore) feeded with 3- or 4- day- old mesophyll protoplasts of Nicotiana tabacum , N. tabacum var. macrophylla and N. rustica and cultured in KM8p basic medium supplemented with various addi-tives at 25℃ in darkness. The zygotes underwent first division after 3d culture and the division frequency was approximately 60%. Microscopical observations showed that the first cell division was unequal in most of the zygotes. Few proembryos and multicellular structures were acquired after 12d culture . The two-celled proembryos also were induced to form multicellular proembryos. We also studied the effects of the isolation method, zy-gote stage and the genotype and age of feeder cells on in vitro development of zygotes." @default.
- W2382431656 created "2016-06-24" @default.
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- W2382431656 date "1998-01-01" @default.
- W2382431656 modified "2023-09-25" @default.
- W2382431656 title "IN VITRO DEVELOPMENT OF ZYGOTES AND TWO-CELLED PROEMBRYOS IN NICOTIANA" @default.
- W2382431656 hasPublicationYear "1998" @default.
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