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- W2382445796 abstract "Objective To study the influence of subculturation on phenotype and genotype of T.rubrum strains.Methods 5 isolated T.rubrum strains(with different phenotypes) and 1 standard strain(ATCC294) were subcultured twenty times on Sabouraud agar slopes.Then we observed the change of phenotypes.Part of the 5.8S DNA and the adjacent ITS2 region,TRS-1 repeat units and TRS-2 repeat units were amplified respectively by PCR from template DNA of the 6 strains and their corresponding twentieth subcultured strains.Then we made nucleotide homology analysis with BLASTN and CLUETER software.Results Phenotype of 6 trains changed many times and could reverse during subculturation.PCR typing and gene sequencing displayed that there were no significant differences between original train and its twentieth one.Conclusion Phenotype of T.rubrum changed and reversed easily during subculturation,the genotype of rDNA was stable relatively." @default.
- W2382445796 created "2016-06-24" @default.
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- W2382445796 date "2007-01-01" @default.
- W2382445796 modified "2023-09-27" @default.
- W2382445796 title "Influence on Phenotype and Ribosomal DNA Sequence of Trichophytin rubrum Resulting from Subculture" @default.
- W2382445796 hasPublicationYear "2007" @default.
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