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- W2382451738 abstract "Taking Lycium barbarum L.as material,primers were designed according to WRKY conserved transcription factor fragment form known species.Lycium barbarum L.WRKY gene fragments were cloned by RT-PCR method.Sequence of the fragments obtained was analyzed.The expression of Lyciumb arbarumL.in various organs was identified by Real-time PCR.They all contributed to research the function of WRKY transcription factor of Lycium barbarum L.The results showed that the cDNA fragment was 467bp and named LbWRKY(GenBank:KF181204).Sequential analysis indicated that the sequence contained WRKYGQK conservative domain,and the similarity with Solanum lycopersicum WRKY and Nicotiana tabacum wizz were 95%and 91%,respectively.The results showed that WRKYgene sequence in Lycium barbarum L.was cloned successfully.Real time PCR analysis showed that the LbWRKYgene was highly expressed in fruit.The expression level reached the lowest level in leaf.LbWRKYdidn't express in root." @default.
- W2382451738 created "2016-06-24" @default.
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- W2382451738 date "2013-01-01" @default.
- W2382451738 modified "2023-09-24" @default.
- W2382451738 title "Cloning and Expression Analysis of WRKY Gene Segment in Lycium barbarumL." @default.
- W2382451738 hasPublicationYear "2013" @default.
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