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- W2382453262 abstract "Objective:Prepare monoclonal antibody(MAb) using SP2/0 cells isolated from tumor in live mouse, and compare the efficiency of cell fusion with conventional method.Methods:The SP2/0 cells were hypodermic injected in the back of the SPF level BALB/c mouse which were 8 week old and re-prepared by isolation from the tumor when it reached 2~3cm diameter. The fusion efficiency of SP2/0 cells from live tumor and from cell culture was compared by fusing with spleen cells. The relative affinity of MAbs made by two methods were also compared.Results:Six fusions have been made. The fusion rate is 70.4% using SP2/0 cells from live tumor, while that is 44.6% using SP2/0 cells from cell culture. The relative affinity of MAbs made by two method were all above 1∶100 000.Conclusion: The fusion efficiency can be significantly improved by fusion spleen cells with SP2/0 cells from live tumor." @default.
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- W2382453262 date "2009-04-20" @default.
- W2382453262 modified "2023-09-26" @default.
- W2382453262 title "Preparation of monoclonal antibody by use of SP2/0 cell from live tumor" @default.
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